Synthesis of C圓-labeled cRNA was performed in ¾ reaction volumes with the ‘Low Input Quick Amp Labeling Kit One-Color’ (#5190-2305, Agilent Technologies) according to the manufacturer’s recommendations.ĬRNA fragmentation, hybridization and washing steps were carried-out as recommended in the ‘One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling Protocol V6.7’, except that 2500ng of labeled cRNA were used for hybridization. RNA isolation was performed based on RNAeasy Micro Kit (#74004, Qiagen) instruction.ġ50ng of total RNA were used as input. aureus Newman strain 2 hours and 24 hours before RNA isolation.Ĭells were maintained in RPMI medium+10%FCS+50ng/ml hM-CSF. GEO help: Mouse over screen elements for information.Ĭells were infected with an MOI 5 of S.
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